gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭

doi:10.11843/j.issn.0366-6964.2018.05.015

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (5): 1005-1011.doi: 10.11843/j.issn.0366-6964.2018.05.015

gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭

赵春芳¹, 李新¹, 韩博¹, 曲鲁江¹, 刘长军², Jiuzhou Song³, 连玲^1*, 杨宁^1*

1. 中国农业大学动物科技学院, 北京 100193;
2. 中国农业科学院哈尔滨兽医研究所禽传染病研究室, 哈尔滨 150001;
3. 马里兰大学帕克分校动物与禽类科学系, 马里兰州 20742, 美国

收稿日期:2017-11-01 出版日期:2018-05-23 发布日期:2018-05-23
通讯作者: 杨宁,教授,E-mail:nyang@cau.edu.cn;连玲,副教授,E-mail:lianlinglara@126.com
作者简介:赵春芳(1989-),女,山东人,博士,主要从事家禽分子遗传育种研究,E-mail:zhyy199002@163.com
基金资助:
863计划（2013AA102501）；国家自然科学基金（31320103905；31301957）；教育部长江学者及创新团队发展计划（IRT_15R62）；现代农业产业技术体系（CARS-41）；青年科学家托举项目；畜禽种质资源平台和青年教师创新计划

gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells

ZHAO Chun-fang¹, LI Xin¹, HAN Bo¹, QU Lu-jiang¹, LIU Chang-jun², SONG Jiu-zhou³, LIAN Ling^1*, YANG Ning^1*

1. Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Division of Avian Infectious Diseases, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;
3. Department of Animal & Avian Sciences, University of Maryland, College Park, Maryland 20742, United States

Received:2017-11-01 Online:2018-05-23 Published:2018-05-23

摘要/Abstract

摘要：

以马立克病病毒（MDV）感染SPF白来航鸡，收集脾和肝来源的淋巴瘤，利用实时荧光定量PCR （qRT-PCR）进行检测，结果显示gga-miR-140-3p在感染MDV的肿瘤化脾和肝淋巴瘤中低表达。为揭示该miRNA在马立克病（MD）肿瘤转化过程中的作用，以该miRNA为研究对象，MDV转化细胞系MDCC-MSB1为试验材料，分别转染miRNA激动剂或阴性对照，检测细胞增殖和迁移，并检测与细胞侵袭密切相关的基因MMP2和MMP9 mRNA表达情况。结果显示，与阴性对照组相比，转染gga-miR-140-3p激动剂后，细胞增殖在24、36和48 h后发生显著（P<0.05）抑制，在60和72 h后发生极显著（P<0.01）抑制。转染激动剂后，细胞迁移数也显著（P<0.05）减少。转染激动剂后，MMP2在转染后24 h转录显著下调（P<0.05），转染后48和72 h转录极显著（P<0.01）下调，MMP9基因在转染后48 h转录显著（P<0.05）下调。gga-miR-140-3p作为在MDV感染组织中异常表达的miRNA，能够影响MD肿瘤淋巴细胞的特征——增殖、迁移和侵袭，提示该miRNA可能参与MD肿瘤转化过程。

Abstract:

Specific pathogen free Leghorn chickens were infected with MDV, and lymphoma from spleens and livers were collected post infection. The qRT-PCR assay showed that microRNA gga-miR-140-3p was downregulated in MDV-induced tumorous spleen and lymphoma from liver. To elucidate the functions of this miRNA in Marek's disease tumor transformation, we transfected MDV-transformed lymphoid cell line, MDCC-MSB1 with gga-miR-140-3p agomir or agomir NC, and inspected their effects on cell proliferation and migration as well as MMP2 and MMP9 genes expression levels whose expression levels were closely correlated with invasion. The proliferation of MSB1 cells decreased at 24, 36, 48, 60 and 72 h post gga-miR-140-3p agomir transfection. The cell number of migration also decreased after transfection with gga-miR-140-3p agomir. MMP2 expression was significantly downregulated at 24, 48 and 72 h; in addition, MMP9 transcription was remarkably downregulated at 48 h post gga-miR-140-3p agomir transfection. In conclusion, gga-miR-140-3p which was downregulated in MDV-induced tumorous tissues had an effect on features of tumor cells such as proliferation, migration and invasion, which indicates that this miRNA may be involved in Marek's disease tumor transformation.

中图分类号:

赵春芳, 李新, 韩博, 曲鲁江, 刘长军, Jiuzhou Song, 连玲, 杨宁. gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭[J]. 畜牧兽医学报, 2018, 49(5): 1005-1011.

ZHAO Chun-fang, LI Xin, HAN Bo, QU Lu-jiang, LIU Chang-jun, SONG Jiu-zhou, LIAN Ling, YANG Ning. gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1005-1011.

参考文献

[1] BIGGS P M. The Leeuwenhoek Lecture,1997.
Marek's disease herpesvirus:oncogenesis and prevention[J].Philos Trans R Soc Lond B Biol Sci, 1997, 352(1364):1951-1962.
[2] CALNEK B W. Marek's disease-a model for herpesvirus oncology[J].Crit Rev Microbiol, 1986, 12(4):293-320.
[3] CALNEK B W. Pathogenesis of Marek's disease virus infection[J].Curr Top Microbiol Immunol, 2001, 255:25-55.
[4] BHATTACHARYA S D, GARRISON J, GUO H T, et al. Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines[J].Surgery, 2010, 148(2):291-297.
[5] XU H T, YAO Y X,SMITH L P,et al.MicroRNA-26a-mediated regulation of interleukin-2 expression in transformed avian lymphocyte lines[J].Cancer Cell Int, 2010, 10:15.
[6] LI X, LIAN L, ZHANG D X, et al.gga-miR-26a targets NEK6 and suppresses Marek's disease lymphoma cell proliferation[J]. Poult Sci, 2014, 93(5):1097-1105.
[7] FAVREAU A J, SATHYANARAYANA P. miR-590-5p,miR-219-5p,miR-15b and miR-628-5p are commonly regulated by IL-3,GM-CSF and G-CSF in acute myeloid leukemia[J].Leuk Res, 2012, 36(3):334-341.
[8] BARTEL D P.MicroRNAs:genomics,biogenesis,mechanism,and function[J]. Cell, 2004, 116(2):281-297.
[9] BRENNECKE J, STARK A, RUSSELL R B, et al. Principles of microRNA-target recognition[J]. PLoS Biol, 2005, 3(3):e85.
[10] YAO Y X, ZHAO Y G,SMITH L P, et al. Differential expression of microRNAs in Marek's disease virus-transformed T-lymphoma cell lines[J]. J Gen Virol, 2009, 90(Pt 7):1551-1559.
[11] ZHAO Y G,YAO Y X,XU H T,et al.A functional MicroRNA-155 ortholog encoded by the oncogenic Marek's disease virus[J]. J Virol, 2009, 83(1):489-492.
[12] CHI J Q, TENG M,YU Z H, et al. Marek's disease virus-encoded analog of microRNA-155 activates the oncogene c-Myc by targeting LTBP1 and suppressing the TGF-β signaling pathway[J].Virology, 2015, 476:72-84.
[13] LI Z J,ZHANG Y P,LI Y,et al.Distinct expression pattern of miRNAs in Marek's disease virus infected-chicken splenic tumors and non-tumorous spleen tissues[J]. Res Vet Sci, 2014, 97(1):156-161.
[14] BURNSIDE J,OUYANG M,ANDERSON A, et al. Deep sequencing of chicken microRNAs[J].BMC Genomics, 2008, 9:185.
[15] LIAN L,LI X,ZHAO C F, et al. Chicken gga-miR-181a targets MYBL1 and shows an inhibitory effect on proliferation of Marek's disease virus-transformed lymphoid cell line[J]. Poult Sci, 2015, 94(11):2616-2621.
[16] LIAN L, QU L J, CHEN Y M, et al. A systematic analysis of miRNA transcriptome in Marek's disease virus-induced lymphoma reveals novel and differentially expressed miRNAs[J].PLoS One, 2012, 7(11):e51003.
[17] LI X,LIAN L,ZHANG D X, et al.gga-miR-26a targets NEK6 and suppresses Marek's disease lymphoma cell proliferation[J]. Poult Sci, 2014, 93(5):1097-1105.
[18] LIAN L,LI X,ZHAO C F,et al.Chicken gga-miR-181a targets MYBL1 and shows an inhibitory effect on proliferation of Marek's disease virus-transformed lymphoid cell line[J]. Poult Sci, 2015, 94(11):2616-2621.
[19] HAN B, LIAN L, LI X, et al.Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek's disease lymphoma cell proliferation and migration[J].Mol Biol Rep, 2016, 43(7):667-676.
[20] ZHAO C F, LI X, HAN B, et al.Gga-miR-219b targeting BCL11B suppresses proliferation,migration and invasion of Marek's disease tumor cell MSB1[J]. Sci Rep, 2017, 7:4247.
[21] TIAN F, LUO J, ZHANG H M, et al. MiRNA expression signatures induced by Marek's disease virus infection in chickens[J].Genomics, 2012, 99(3):152-159.
[22] AKIYAMA Y, KATO S. Two cell lines from lymphomas of Marek's disease[J].Biken J, 1974, 17(3):105-116.
[23] NAZERIAN K. An updated list of avian cell lines and transplantable tumours[J].Avian Pathol, 1987, 16(3):527-544.
[24] TAN X G, QIN W Y, ZHANG L, et al. A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis[J].Clin Cancer Res, 2011, 17(21):6802-6811.
[25] MILES G D, SEILER M, RODRIGUEZ L, et al. Identifying microRNA/mRNA dysregulations in ovarian cancer[J].BMC Res Notes, 2012, 5:164.
[26] SAND M,SKRYGAN M,GEORGAS D, et al. Microarray analysis of microRNA expression in cutaneous squamous cell carcinoma[J].J Dermatol Sci, 2012, 68(3):119-126.
[27] SAND M,SKRYGAN M, SAND D, et al. Expression of microRNAs in basal cell carcinoma[J].Br J Dermatol, 2012, 167(4):847-855.
[28] ZOU M X,HUANG W,WANG X B, et al. Identification of miR-140-3p as a marker associated with poor prognosis in spinal chordoma[J]. Int J Clin Exp Pathol, 2014, 7(8):4877-4885.
[29] TIAN F, LUO J, ZHANG H M, et al. MiRNA expression signatures induced by Marek's disease virus infection in chickens[J].Genomics, 2012, 99(3):152-159.
[30] LIAN L, ZHANG D X, WANG Q, et al. The inhibitory effects of gga-miR-199-3p,gga-miR-140-3p,and gga-miR-221-5p in Marek's disease tumorigenesis[J].Poult Sci, 2015, 94(9):2131-2135.
[31] BURNSIDE J, OUYANG M, ANDERSON A, et al. Deep sequencing of chicken microRNAs[J]. BMC Genomics, 2008, 9:185.
[32] SONG B, WANG Y, XI Y, et al. Mechanism of chemoresistance mediated by miR-140 in human osteosarcoma and colon cancer cells[J].Oncogene, 2009, 28(46):4065-4074.
[33] GU R,SUN Y F, WU M F, et al. Biological roles of microRNA-140 in tumor growth, migration, and metastasis of osteosarcoma in vivo and in vitro[J].Tumour Biol, 2016, 37(1):353-360.
[34] KONG X M, ZHANG G H, HUO Y K, et al. MicroRNA-140-3p inhibits proliferation, migration and invasion of lung cancer cells by targeting ATP6AP2[J]. Int J Clin Exp Pathol, 2015, 8(10):12845-12852.
[35] DONG W, YAO C P, TENG X P, et al. MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer[J].Tumour Biol, 2016, 37(3):2973-2985.
[36] STETLER-STEVENSON W G, AZNAVOORIAN S,LIOTTA L A. Tumor cell interactions with the extracellular matrix during invasion and metastasis[J].Annu Rev Cell Biol, 1993, 9(1):541-573.
[37] SUN J, HEMLER M E. Regulation of MMP-1 and MMP-2 production through CD147/extracellular matrix metalloproteinase inducer interactions[J]. Cancer Res, 2001, 61(5):2276-2281.
[38] LUO Y,LIANG F B,ZHANG Z Y.PRL1 promotes cell migration and invasion by increasing MMP2 and MMP9 expression through Src and ERK1/2 pathways[J]. Biochemistry, 2009, 48(8):1838-1846.
[39] TABOURET E,BOUDOURESQUE F,FARINA P,et al.MMP2 and MMP9 as candidate biomarkers to monitor bevacizumab therapy in high-grade glioma[J].Neuro Oncol, 2015, 17(8):1174-1176.

gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭

gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells

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